The methylated and low-methylated matched pair DNA can be utilized as positive and negative controls,respectively, in research focusing on gene methylation studies. The low-methylated genomic DNAs are selected from human young donor blood peripheral leukocyte, liver tissue, or lymphocyte cell line. The DNAs derived from these sources naturally have a low level of methylation and display batch-to-batch consistency. Unlike DNAs from the DNA methyltransferase genetic knockout cell lines, these DNA sources are ideal for more natural control DNAs to be used in gene methylation studies.